Splice
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The splice() method changes the contents of an array by removing or replacing existing elements and/or adding new elements in place. To access part of an array without modifying it, see slice().
The splice() method is a mutating method. It may change the content of this. If the specified number of elements to insert differs from the number of elements being removed, the array's length will be changed as well. At the same time, it uses @@species to create a new array instance to be returned.
The splice() method is generic. It only expects the this value to have a length property and integer-keyed properties. Although strings are also array-like, this method is not suitable to be applied on them, as strings are immutable.
\\n The splice() method changes the contents of an array by\\n removing or replacing existing elements and/or adding new elements in place. To access part of an array without modifying it, see slice().\\n
Splice is an experimental and artistic puzzler. Immerse yourself in its microbial world and start splicing! Every level (\"strand\") consists of a number of cells that you will need to rearrange into a target structure in several moves (\"splices\"). Exploration and experimentation are key in determining how the curious little cells react to each other.
Removes the elements designated by OFFSET and LENGTH from an array, and replaces them with the elements of LIST, if any. In list context, returns the elements removed from the array. In scalar context, returns the last element removed, or undef if no elements are removed. The array grows or shrinks as necessary. If OFFSET is negative then it starts that far from the end of the array. If LENGTH is omitted, removes everything from OFFSET onward. If LENGTH is negative, removes the elements from OFFSET onward except for -LENGTH elements at the end of the array. If both OFFSET and LENGTH are omitted, removes everything. If OFFSET is past the end of the array and a LENGTH was provided, Perl issues a warning, and splices at the end of the array.
Training set: Our training and test sets of human and Drosophila melanogaster splice sites are available to the community for testing splice site predictors. They can be obtained from our collection of representative, standardized data sets of human and D. melanogaster genes.
The N6-methyladenosine (m6A) RNA modification is used widely to alter the fate of mRNAs. Here we demonstrate that the C. elegans writer METT-10 (the ortholog of mouse METTL16) deposits an m6A mark on the 3' splice site (AG) of the S-adenosylmethionine (SAM) synthetase pre-mRNA, which inhibits its proper splicing and protein production. The mechanism is triggered by a rich diet and acts as an m6A-mediated switch to stop SAM production and regulate its homeostasis. Although the mammalian SAM synthetase pre-mRNA is not regulated via this mechanism, we show that splicing inhibition by 3' splice site m6A is conserved in mammals. The modification functions by physically preventing the essential splicing factor U2AF35 from recognizing the 3' splice site. We propose that use of splice-site m6A is an ancient mechanism for splicing regulation.
Now that we have an array that we can mutate on, we can iterate through every item in the first array. For each item in the first array we can use the splice() function to insert the item into index n of localArray.
Modern, strong, high modulus fibers are often slippery and cannot be secured with customarily trustworthy knots and splices. These fibers encouraged the development of the loosely woven, hollow braid ropes in which the fiber-alignment maximized strength. These ropes require suitable splicing techniques such as the Brummel and Long Bury. The ropes yield excellent strength from the fibers, and the splices weaken the ropes hardly at all.
While the result is the same, what is going on under the hood iscompletely different. list2() is a dots collector thatspecial-cases !!! arguments. On the other hand, inject()operates on the language and creates a function call containing asmany arguments as there are elements in the spliced list. If yousupply a list of size 1e6, inject() is creating one millionarguments before evaluation. This can be much slower.
Comprehensive analysis of splice variation is limited with short-read sequencing; although exon junctions can be observed, resolution of entire isoforms is extremely challenging. With nanopore sequencing, read length is equal to fragment length, meaning entire transcripts can be sequenced in single reads. Full-length transcripts >20 kb in length have been sequenced in single reads. This greatly simplifies the identification and quantification of entire isoforms (Figure 2).
Genetic variation within the gene CACNA1C, encoding the voltage-gated calcium channel CaV1.2, is associated with neuropsychiatric disorders, including schizophrenia and bipolar disorder. However, the basis for the underlying genetic association is unknown. Using long-range PCR and nanopore cDNA sequencing of full-length CACNA1C transcripts, Clark et al. performed an in-depth analysis of its splice variants in post-mortem human brain tissue. This investigation revealed the true complexities of CACNA1C splicing: 38 novel exons were observed, and 241 of 251 total transcripts identified were novel. Many of the novel transcripts were found to be abundantly expressed and encode for aberrant protein products with altered function. The researchers state that such detailed results help to advance our understanding of these neuropsychiatric disorders, and provide potential pharmacological targets.
Oxford Nanopore provides three RNA sequencing kits that can be used for gene expression and downstream splice variation analysis, all of which deliver full-length transcripts. The choice of kit depends on your specific study requirements, including sample amounts, requirement for sample multiplexing, base modification detection, and desired number of reads.
The range of nanopore sequencing platforms enables you to scale according to your throughput and output requirements, from the portable Flongle and MinION devices, which are well suited to targeted splice variation analysis, to the modular GridION and ultra-high-throughput PromethION P24/48 platforms, ideal for transcriptome-wide investigations.
Library preparation with the Direct cDNA Sequencing Kit, followed by sequencing on the PromethION device, delivers 15-30 million reads per flow cell, ideal for transcriptome-wide analysis of splice variation. Sample multiplexing can be achieved using the Native Barcoding Expansion Packs.
The JavaScript Array splice() Method is an inbuilt method in JavaScript that is used to modify the contents of an array by removing the existing elements and/or by adding new elements.
Sounds like a network fs that uses splice needs to have acknowledgements of when data is received, at the network fs protocol level. Not familiar with SMB, but I guess it lacks SMB level of signalling of file transfer completion. Rethinking splice() Posted Feb 17, 2023 16:48 UTC (Fri) by wtarreau (subscriber, #51152) [Link]
splice() should have been modified to use just regular descriptors. E.g. if I want to connect a file descriptor to a network socket, I should just do it directly. This way the kernel can have special-cased handling of file-based pages and provide meaningful completion notifications. There's probably a handful of such combinations that make sense (memfd() to socket, socket to memfd() or file, etc.)To make it even better, add a flag F_SPLICE_NO_FALLBACK that will fail the operation if there's no accelerated path available and the kernel would instead just fall back on a memcpy. Rethinking splice() Posted Feb 18, 2023 6:40 UTC (Sat) by NYKevin (subscriber, #129325) [Link]
At this point, it's even worse than setuid/seteuid/etc., and that mess led directly to the creation of setresuid(2). Why can't we (application programmers) have nice things once in a while This is not O_PONIES. This is a simple matter of \"don't make three different syscalls that do basically the same thing.\" Or maybe even \"don't provide abstractions that leak like a sieve.\"All we need is one, single syscall that:* Is exactly equivalent to a while/read/write loop.* Starts reading/writing from wherever the fd is currently positioned (if it's seekable and you want to seek, then call seek explicitly).* Takes a size argument (which is just about the only thing all three of those syscalls have in common).* Fails with EDOITYOURSELF if there's no optimization available and the kernel doesn't feel like emulating it. Then libc or somebody else can write a simple wrapper that does the while loop if necessary.* Blocks at least until the last write (in a hypothetical while/read/write loop) would have returned. It might still need to be fsync'd, but there should be no \"oh, if you do a write at exactly the wrong time, it'll silently clobber all of your data\" case.* Also, O_NONBLOCK and/or io_uring would be nice to have, but *now* we're getting more into the O_PONIES realm, so I would say this is a bonus goal (but still probably doable). Rethinking splice() Posted Feb 18, 2023 6:56 UTC (Sat) by NYKevin (subscriber, #129325) [Link]
To be clear: When I say this, I'm referring to the application-observable semantics of the operation, not to the underlying mechanism. In other words, I don't care whether the kernel actually copies the data, or moves it, or COWs it, or makes it dance the moonwalk. All I care about is the *semantics* - when this hypothetical syscall returns, it means the kernel considers the operation \"committed\" in some sense, and I can now proceed to close the src, or reopen it as writable and scribble on it, or truncate it, or whatever else I feel like, and the dest will not get messed up. Rethinking splice() Posted Feb 20, 2023 7:38 UTC (Mon) by moxfyre (guest, #13847) [Link] 59ce067264
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